EPA Method 300.1

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EPA Method 300.1:
Anions in Water by IC. Official Name: Determination of Inorganic Anions in Drinking Water by Ion Chromatography

The concentration of anions in a 10-uL (for common anions) or 50-uL (for disinfection byproducts) sample are determined using an ion chromatography (IC) system equipped with a conductivity detector (CD).

This method determines certain inorganic anions (both common and disinfection byproducts) in reagent water, surface water, ground water, and finished drinking water. Methyl fluoroacetate is measured using the fluoride ion.

Methods for the Determination of Organic and Inorganic Compounds in Drinking Water, Volume 1 (EPA/815-R-00-014)

(A) Co-elution: Modifying the eluent with organic solvents, changing the detection system, or pretreating the sample can reduce co-elution.(B) Pretreatment cartridges: Use of a pretreatment cartridge can lead to artifacts in the sample; use with caution.(C) Turbidity: Remove turbidity using filtration.(D) Weakly-bound anions: Anions which are weakly retained by the column interfere with fluoride determination at > 1.5 mg/L.(E) Carryover effects: There are some compounds in ozonated and chlorine dioxide matrices which will elute well after sulfate. These may carry over in later runs.(F) Chlorine dioxide. Chlorine dioxide can form chlorite. Remove chlorine dioxide by purging the sample with inert gas.

QC Requirements:
Initial demonstration of laboratory performance and subsequent analysis in each analysis batch of a Laboratory Reagent Blank (LRB), Laboratory Fortified Blank (LFB), Instrument Performance Check Standard (IPC), Calibration Check Standards, Laboratory Fortified Sample Matrices (LFM), and either Field, Laboratory or LFM duplicate sample analyses.

Maximum Holding Time:
28 days for bromide, chloride, fluoride, bromate, and chlorate14 days for chlorite48 hours for Nitrate-N, Nitrite-N, and ortho-Phosphate-P



Linear calibration range (LCR) should be established by the analyst.

Precision and accuracy values were calculated using data from nine replicate analyses of a fortified reagent water sample. Additional precision and accuracy data, including data from different matrices, are available in the method.

MDL procedure is equivalent to the procedure at 40 CFR part 136, Appendix B. MDLs were determined using the standard deviation of 7 replicate analyses of an analyte-fortified reagent water sample multiplied by the t-value for 6 degrees of freedom at the 99% confidence level (3.14). Detailed instructions for how MDLs are determined are found in Section 9.2.4 of the Method.

Revision Number:
Revision 1.0, 1997

Test Description:
Inorganic Anions in

Report Number:

Instrument used for this test: