EPA Method 1002

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EPA Method 1002:
Survival and Reproduction Test using Daphnids. Official Name: EPA Test Method 1002.0: Daphnid, Ceriodaphnia dubia, Survival and Reproduction Test

Ceriodaphnia dubia are exposed in a static renewal system to different concentrations of effluent, or to receiving water, until 60% or more of surviving control females have three broods of offspring. Test results are based on survival and reproduction. If the test is conducted as described, the surviving control organisms should produce 15 or more young in three broods. If these criteria are met at the end of 8 days, the test must be repeated.

This method measures the chronic toxicity of effluents and receiving waters to the daphnid, Ceriodaphnia dubia, using less than 24h old neonates during a three-brood (seven-day), static renewal test. The effects include the synergistic, atagonistic, and additive effects of all the chemical, physical, and biological components which adversely affect the physiological and biochemical functions of the test organisms.

Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms

Introduced toxic substances; Low D.O.; High concentrations of suspended and/or dissolved solids; Pathogenic and/or predatory organisms

QC Requirements:
Reference toxicant testing with control charts for each combination of toxicant, test species, test condition, and endpoint.

Maximum Holding Time:


Chronic Toxicity

Effluents: 5 and a control Receiving waters: 100% receiving water (or minimum of 5) and a control (recommended)

Sample Prep:
Filtration, Dechlorination.

It is a laboratory's responsibility to demonstrate its ability to obtain consistent, precise results with reference toxicants before it performs toxicity tests with effluents for permit compliance purposes. To meet this requirement, the intra-laboratory precision, expressed as percent coefficient of variation, of each type of test to be used in a laboratory should be determined by performing five or more tests with different batches of test organisms using the same reference toxicant, at the same concentrations, with the same test conditions, and the same data analysis methods.


Revision Number:
Fourth Edition, October 2002