EPA Method 1614

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EPA Method 1614:
Brominated Diphenyl Ethers in Water Soil, Sediment and Tissue by HRGC/HRMS

Summary:
Aqueous samples (samples containing less than one percent solids)-Stable isotopically labeled analogs of the BDEs are spiked into a 1-L sample. The sample is extracted using solid-phase extraction (SPE), separatory funnel extraction (SFE), or continuous liquid/liquid extraction (CLLE). Solid, semi-solid, and multi-phase samples (excluding tissue)-The labeled compounds are spiked into a sample containing 10 g (dry weight) of solids. Samples containing multiple phases are pressure filtered and any aqueous liquid is discarded. Coarse solids are ground or homogenized. Any non-aqueous liquid from multi-phase samples is combined with the solids and extracted in a Soxhlet/Dean-Stark extractor. Fish and other tissue-A 20-g aliquot of sample is homogenized, and a 10-g aliquot is spiked with the labeled compounds. The sample is mixed with anhydrous sodium sulfate, dried for a minimum of 30 minutes, and extracted for 18-24 hours using methylene chloride in a Soxhlet extractor. The extract is evaporated to dryness, and the lipid content is determined.

Scope:
EPA Method 1614 ("Method 1614"; the "Method") is for determination of brominated diphenyl ether (BDE) congeners in water, soil, sediment, biosolids, tissue, and other sample matrices by high resolution gas chromatography combined with high resolution mass spectrometry (HRGC/HRMS).