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View Actual EPA Method 1632A (PDF File)
EPA Method 1632A:Chemical Speciation of Arsenic in Water and Tissue by Hydride Generation Quartz Furnace Atomic Absorption Spectrometry
Summary:Aqueous sample- A 500- to 1000-mL water sample is collected directly into a cleaned fluoropolymer, conventional or linear polyethylene, polycarbonate, or polypropylene sample bottle using sample handling techniques specially designed for collection of metals at trace levels (Reference 16.3). Water samples are preserved in the field by the addition of 3 mL of pretested 6M HCl per liter of sample. The recommended holding time is 28 days.
Scope:This method is for determination of inorganic arsenic (IA), arsenite (As +3 ), arsenate (As +5 ,( monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) in filtered and unfiltered water and in tissue by hydride generation and quartz furnace atomic absorption detection. The method is for use in EPA's data gathering and monitoring programs associated with the Clean Water Act. The method is based on a contractor-developed method (Reference 16.1) and on peer-reviewed, published procedures for the speciation of As in aqueous samples (Reference 16.2)
Interferences:Preventing ambient water samples from becoming contaminated during the sampling and analytical processes constitutes one of the greatest difficulties encountered in trace metal determinations. Over the last two decades, marine chemists have come to recognize that much of the historical data on the concentrations of dissolved trace metals in seawater are erroneously high because the concentrations reflect contamination from sampling and analysis rather than ambient levels. Therefore, it is imperative that extreme care be taken to avoid contamination when collecting and analyzing ambient water samples for As species at trace levels