EPA Method 1680

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EPA Method 1680:
Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC Medium

Method 1680 provides for the enumeration of fecal coliforms in Class A and Class B biosolids using the most probable number (MPN) procedure. In Method 1680, LTB is used as a presumptive medium followed by EC as confirmation of fecal coliforms. EC may not be used for direct isolation from a biosolid sample because prior enrichment in presumptive medium (LTB) is required for optimum recovery of fecal coliforms.

This method describes multiple-tube fermentation procedures [also called the most probable number (MPN) procedure] for the detection and enumeration of fecal coliform bacteria in biosolids. These methods use culture-specific media and elevated temperature to isolate and enumerate fecal coliform organisms. Fecal coliform bacteria, including Escherichia coli (E. coli), are commonly found in the feces of humans and other warm-blooded animals, and indicate the potential presence of other bacterial and viral pathogens.

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