EPA Method 2019

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EPA Method 2019:
Acute Toxicity Tests for Waters Using Rainbow Trout and Brook Trout. Official Name: EPA Test Method 20019.0: Rainbow Trout, Oncorhynchus mykiss, and Brook Trout, Salvelinus fontinalis, Acute Toxicity Tests with Effluents and Receiving Waters

Early life stage Oncorhynchus mykiss and Salvelinus fontinalis are exposed to varying concentrations of effluent or receiving water in a static non-renewal, static-renewal, or flow-through system for a pre-determined amount of time (24, 48, or 96 h). The survival in each concentration is compared to the survival in the control to determine the concentration of effluent or receiving water lethal to 50% of test organisms.

This procedure describes an acute toxicity test method for use in the National Pollutant Discharge Elimination System (NPDES) Permits Program to identify effluents and receiving waters containing toxic materials in acutely toxic concentrations. This method is also suitable for determining the toxicity of specific compounds contained in discharges. This test may be conducted in a central laboratory or on-site, by the regulatory agency or the permittee.

Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms. Fifth Edition, October 2002. EPA Volume 821-R-02-012


QC Requirements:
Routine reference toxicant testing

Maximum Holding Time:


Acute Toxicity

Effluents: 5 and a control (minimum) Receiving Water: 100% receiving water and a control (recommended)

Sample Prep:
Coarse filtration; Dechlorination

Based on single and multi-laboratory precision data.


Revision Number:
Fifth Edition, October 2002