EPA Method 350.1

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EPA Method 350.1:
Ammonia by Automated Colorimetry. Official Name: Nitrogen, Ammonia (Colorimetric, Automated Phenate)

Summary:
Alkaline phenol and hypochlorite react with ammonia to form indophenol blue that is proportional to the ammonia concentration. The blue color is intensified with sodium nitroprusside.

Scope:
This method determines ammonia in drinking, surface, and saline waters; domestic and industrial wastes.

Citation:
Methods for the Determination of Inorganic Substances in Environmental Samples (EPA/600/R-93/100)

Interferences:
(A) Calcium and Magnesium: Ions of these metals can cause errors by precipitating during analyses. Use EDTA solution (for river and industrial waters) or potassium tartrate solution (for sea water) to reduce this interference.(B) Turbidity: Sample turbidity should be removed by filtration.(C) Color: Color absorbing in the range of the indophenol blue complex can interfere.

QC Requirements:
None.

Maximum Holding Time:
28 days (MCAWW, Table 1).

Media:
WATER

Subcategory:
Inorganic

Concentration:
0.01 - 2.0 mg/L (ammonia as N)

Sample Prep:
None.

Precision:
Precision and accuracy values were calculated using interlaboratory data from EPA-managed Water Pollution (WP) performance evaluation studies.

Detection:
None.

Revision Number:
Revision 2.0. August 1993

Test Description:
Ammonia (as nitrogen

Report Number:
600/4-79-020

Instrument used for this test:
FIA