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View Actual EPA Method 353.1 (PDF File)
EPA Method 353.1:Nitrate-Nitrite by Colorimetry. Official Name: Nitrogen, Nitrate-Nitrite (Colorimetric, Automated Hydrazine Reduction)
Summary:Nitrate in a sample is reduced to nitrite using hydrazine sulfate and the nitrite in the sample (originally present plus reduced nitrate) is determined by diazotizing with sulfanilamide and coupling with N-(1-naphthyl)-ethylenediamine dihydrochloride to form a highly colored azo dye which is measured colorimetrically. This automated procedure can measure approximately 20 samples per hour.
Scope:This method determines nitrate and nitrite in drinking and surface water; domestic and industrial wastes.
Citation:Methods for the Chemical Analysis of Water and Wastes (MCAWW) (EPA/600/4-79/020)
Interferences:(A) Color: Sample color that absorbs in the range used for analysis will interfere.(B) Sulfide: Nitrate and nitrite concentrations can vary up to 10% with concentration of sulfide ions up to 10 mg/L.
QC Requirements:None.
Maximum Holding Time:28 Days (nitrate+nitrite)(MCAWW, Table 1).
Media:WATER
Subcategory:Inorganic
Concentration:0.01 - 10 mg/L nitrate-nitrite nitrogen
Sample Prep:None.
Precision:Precision and accuracy values were calculated using interlaboratory data from EPA-managed Water Pollution (WP) performance evaluation studies.
Detection:None.
Revision Number:Issued 1971; Reissued with Revision 1978
Test Description:Total Inorganic Nitrogen (Calc)