EPA Method 353.3

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EPA Method 353.3:
Nitrate-Nitrite by Cadmium Reduction and Colorimetry. Official Name: Nitrogen, Nitrate-Nitrite (Spectrophotometric, Cadmium Reduction)

Summary:
Nitrate and nitrite: A filtered sample is passed through a column containing granulated copper-cadmium to reduce nitrate to nitrite. The nitrite (originally in the sample and reduced nitrate) is determined by diazotizing with sulfanilamide and coupling with N-(1-naphthyl)-ethylenediamine dihydrochloride to form a highly colored azo dye, which is measured with a spectrometer.Nitrite alone: The procedure is the same except that the cadmium column is bypassed.

Scope:
This method determines nitrite singly or nitrate and nitrite combined in drinking, surface, and saline waters, domestic and industrial wastes.

Citation:
Methods for the Chemical Analysis of Water and Wastes (MCAWW) (EPA/600/4-79/020)

Interferences:
(A) Suspended matter: Suspended materials will fowl the reduction column and restrict sample flow. Pretreat by filtering the sample through a 0.45 um filter or (for highly turbid samples) pretreating with zinc sulfate before filtration.(B) Metals: Metals like iron or copper can produce low results. Treat samples with EDTA to remove this interference.(C) Oil and grease: Oil and grease, that can coat the column and reduce efficiency should be removed by pre-extracting the sample with an organic solvent.

QC Requirements:
None.

Maximum Holding Time:
28 Days (nitrate+nitrite)48 hours (nitrate or nitrite, singly)(MCAWW, Table 1).

Media:
WATER

Subcategory:
Inorganic

Concentration:
0.01 to 1.0 mg/L Nitrate-nitrite nitrogen.

Sample Prep:
None.

Precision:
Precision and accuracy values were calculated using interlaboratory data from EPA-managed Water Pollution (WP) performance evaluation studies.

Detection:
None.

Revision Number:
Issued 1974

Test Description:
Nitrate-Nitrite (as

Report Number:
600/4-79-020