EPA Method 3640A

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EPA Method 3640A:
GEL-PERMEATION CLEANUP

Summary:
The column is packed with the required amount of preswelled absorbent, and is flushed with solvent for an extended period. The column is calibrated and then loaded with the sample extract to be cleaned up. Elution is effected with a suitable solvent(s) and the product is then concentrated.

Scope:
Gel-permeation chromatography (GPC) is a size exclusion cleanup procedure using organic solvents and hydrophobic gels in the separation of synthetic macromolecules (1). The packing gel is porous and is characterized by the range or uniformity (exclusion range) of that pore size. In the choice of gels, the exclusion range must be larger than the molecular size of the molecules to be separated (2). A cross-linked divinylbenzene-styrene copolymer (SX-3 Bio Beads or equivalent) is specified for this method.

Report Number:
SW-846 Ch 4.2.2