EPA Method 4035

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EPA Method 4035:
SOIL SCREENING FOR POLYNUCLEAR AROMATIC HYDROCARBONS BY IMMUNOASSAY

Summary:
An accurately weighed sample is first extracted and the extract filtered using a commercially available test kit. The sample extract and an enzyme conjugate reagent are added to immobilized antibody. The enzyme conjugate "competes" with the PAHs present in the sample for binding to the immobilized anti-PAH antibody. The test is interpreted by comparing the response produced by testing a sample to the response produced by testing standard(s) simultaneously.

Scope:
Method 4035 is a procedure for screening soils to determine when total polynuclear aromatic hydrocarbons (PAHs) are present at concentrations above 1 mg/kg. Method 4035 provides an estimate for the concentration of PAHs by comparison with a PAH standard.

Report Number:
SW-846 Ch 4.4