EPA Method 4040

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EPA Method 4040:

Test kits are commercially available for this method. The manufacturer's directions should be followed. In general, the method is performed using an extract of a soil sample. Filtered extracts may be stored cold, in the dark. An aliquot of the extract and an enzyme-toxaphene conjugate reagent are added to an immobilized toxaphene antibody. The enzyme-toxaphene conjugate "competes" with toxaphene present in the sample for binding to the immobilized toxaphene antibody. The enzyme-toxaphene conjugate bound to the toxaphene antibody then catalyzes a colorless substrate to a colored product. The test is interpreted by comparing the color produced by a sample to the response produced by a reference reaction.

Method 4040 is a procedure for screening soils to determine whether toxaphene (CAS Registry 8001-35-2) is present at concentrations above 0.5 ?g/g. Method 4040 provides an estimate for the concentration of toxaphene by comparison against standards.

Report Number:
SW-846 Ch 4.4