EPA Method 4041

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EPA Method 4041:
SOIL SCREENING FOR CHLORDANE BY IMMUNOASSAY

Summary:
Test kits are commercially available for this method. The manufacturer's directions should be followed. In general, the method is performed using an extract of a soil sample. Filtered extracts may be stored cold, in the dark. An aliquot of the extract and an enzyme-chlordane conjugate reagent are added to immobilized chlordane antibody. The enzyme-chlordane conjugate "competes" with chlordane present in the sample for binding to chlordane antibody. The enzyme-chlordane conjugate bound to the chlordane antibody then catalyzes a colorless substrate to a colored product. The test is interpreted by comparing the color produced by a sample to the response produced by a reference reaction.

Scope:
Method 4041 is a procedure for screening soils to determine whether chlordane (CAS Registry 57-74-9) is present at concentrations above 20, 100 or 600 ?g/kg. Method 4041 provides an estimate for the concentration of chlordane by comparison against standards.

Report Number:
SW-846 Ch 4.4