EPA Method 405.1

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EPA Method 405.1:
Biochemical Oxygen Demand. Official Name: Biochemical Oxygen Demand (5 days, 20oC)

Summary:
The BOD test is an empirical bioassay-type test which measures the dissolved oxygen consumed by microbial life while assimilating and oxidizing organic matter in a sample. A waste sample (or dilution) is incubated for five days 20oC in the dark. Dissolved oxygen is measured before and after incubation using a modified Winkler or oxygen probe method (e.g., EPA Method 360.2 and 360.1). The reduction in dissolved oxygen during the incubation period yields a measure of BOD.

Scope:
This test for BOD is used to determine the relative oxygen requirements of municipal and industrial wastewaters.

Citation:
Methods for the Chemical Analysis of Water and Wastes (MCAWW) (EPA/600/4-79/020)

Interferences:
None Provided.

QC Requirements:
None.

Maximum Holding Time:
48 hours (MCAWW, Table 1).

Media:
WATER

Subcategory:
Organic

Concentration:
See EPA Methods 360.1 and 360.2 for general information on the range of dissolved oxygen methods.

Sample Prep:
None.

Precision:
Precision and accuracy values were calculated using interlaboratory data from EPA-managed Water Pollution (WP) performance evaluation studies.

Detection:
None.

Revision Number:
Issued 1971; Editroial Revision 1974

Test Description:
Biochemical Oxygen D

Report Number:
600/4-79-020

Instrument used for this test:
DOMeter