EPA Method 4051

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EPA Method 4051:
HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE (RDX) IN SOIL BY IMMUNOASSAY

Summary:
Test kits are commercially available for this method. The manufacturer's directions should be followed. In general, the method is performed using an extract of a soil sample. Samples and an enzyme conjugate reagent are added to immobilized RDX antibody. The enzyme-RDX conjugate "competes" with RDX present in the sample for binding to an immobilized RDX antibody. The enzyme-RDX conjugate bound to the antibody then catalyzes a colorless substrate to a colored product. The test is interpreted by comparing the color produced by a sample to the response produced by a reference reaction.

Scope:
Method 4051 is a procedure for screening soils to determine when hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX, CAS No. 121-82-4) is present at concentrations above 0.5 mg/kg Method 4051 provides an estimate of the concentration of RDX by comparison with a reference.

Report Number:
SW-846 Ch 4.4