EPA Method 532

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EPA Method 532:
Determination of Phenylurea Compounds in Drinking Water by Solid Phase Extraction and High Performance Liquid Chromatography with UV Detection.

A 500-mL sample is extracted using a solid phase extraction (SPE) cartridge or disk. The pesticides are elute from the SPE cartridge or disk using methanol, and the methanol extract is concentrated. The concentrations of pesticides in the extract are measured using a high performance liquid chromatography (HPLC) system equipped with an ultraviolet/visible (UV/Vis) detector.

This method determines phenylurea pesticides in drinking water.

Methods for the Determination of Organic and Inorganic Compounds in Drinking Water, Volume 1 (EPA/815-R-00-014)

(A) Glassware contamination: Solvent rinse and bake glassware to minimize contamination.(B) Co-extracted interferences: Compounds that are co-extracted with analytes may interfere. A peak is observed near fluometuron which can interfere with quantitation.(C) Contamination: Solid Phase Extraction (SPE) devices can introduce contaminants.

QC Requirements:
Initial Demonstration of Capability (IDC), determination of the MDL, subsequent analysis in each batch of a Laboratory Reagent Blank (LRB), Continuing Calibration Check Standards (CCC), a Laboratory Fortified Blank (LFB), a Laboratory Fortified Sample Matrix (LFM), and either a Laboratory Fortified Sample Matrix Duplicate (LFMD) or a Field Duplicate Sample.

Maximum Holding Time:
14 days; 21 days after extraction (Samples exchanged into reagent water/acetonitrile (60/40) for confirmational analysis may be stored 7 days at 0oC or less; however, the combined extract holding time may not exceed 21 days.)



1.0-30.0 ug/L for all analytes.

Precision and accuracy values were calculated using data from the analyses of seven replicate fortified reagent water samples in a single laboratory using conditions specified in Table 4 of the method. Additional data are available in the method.

MDLs represent performance on the primary column. Secondary columns used in validation of the method allowed for much lower MDLs for some analytes. Consult the method for more information. MDLs were determined using the standard deviation of 7 replicate analyses of a analyte-fortified reagent water (over a 3-day span) multiplied by the t-value for 6 degrees of freedom at the 99% confidence level. Detailed instructions for how MDLs are determined are found at 40 CFR part 136, Appendix B and Section 9.2.4 of the method.

Revision Number:
Revision 1.0, June 2000