EPA Method 535

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EPA Method 535:
MEASUREMENT OF CHLOROACETANILIDE AND OTHER ACETAMIDE HERBICIDE DEGRADATES IN DRINKING WATER BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (LC/MS/MS)

Summary:
Analytes and surrogate are extracted and concentrated by passing a 250-mL water sample through a solid phase extraction (SPE) cartridge containing 0.5 g of nonporous graphitized carbon. The compounds are eluted from the solid phase with a small quantity of methanol containing 10 mM ammonium acetate. The methanol extract is then concentrated to dryness and reconstituted with 1 mL of water containing 5 mM ammonium acetate. The analytes are chromatographically separated by injecting an aqueous aliquot (100 ?L) into an LC system equipped with a reversed phase (C18) column. After elution from the column, the analytes are detected by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The concentration of each identified component is measured by an internal standard procedure, i.e. relating the product ion response of that compound to the product ion response of the compound that is used as an internal standard. A surrogate analyte, whose concentration is known in every sample, is measured with the same internal standard calibration procedure.

Scope:
This is a liquid chromatographic (LC)/tandem mass spectrometric (MS/MS) method applicable to the determination of the ethanesulfonic acid (ESA) and oxanilic acid (OA) degradates of the chloroacetanilide and other acetamide herbicides in finished drinking waters. The single laboratory Lowest Concentration Minimum Reporting Level (LCMRL) has also been determined in reagent water.

Test Description:
Chloroacetanilide and other Acetamide Herbicide Degradates in DW by Solid Phase Extrn and LC/MS/MS

Instrument used for this test:
HPLC-MS