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View Actual EPA Method 538 (PDF File)
EPA Method 538:DETERMINATION OF SELECTED ORGANIC CONTAMINANTS IN DRINKING WATER BY DIRECT AQUEOUS INJECTION-LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (DAI-LC/MS/MS)
Summary:A 40-mL water sample is collected in a bottle containing sodium omadine and ammonium acetate. An aliquot of the sample is placed in an autosampler vial and the internal standards are added. A 50-?L or larger injection is made into an LC equipped with a C18 column that is interfaced to an MS/MS operated in the electrospray ionization (ESI) mode. The analytes are separated and identified by comparing the acquired mass spectra and retention times to reference spectra and retention times for calibration standards acquired under identical LC/MS/MS conditions. The concentration of each analyte is determined by internal standard calibration using procedural standards.
Scope:This is a direct aqueous injection-liquid chromatography/tandem mass spectrometry (DAI-LC/MS/MS) method for the determination of selected nonvolatile chemical contaminants in drinking water.