EPA Method 547

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EPA Method 547:
Determination of Glyphosate in Drinking Water by Direct-Aqueous-Injection b HPLC, Post-Column Derivatization, and Fluorescence Detection - July 1990.

An aliquot of a filtered water sample is injected into a cation exchange high performance liquid chromatography (HPLC) column. Separation is achieved by using an isocratic elution. After elution from the analytical column, the analyte is oxidized with calcium hypochlorite. The product (glycine) is then coupled with o-phthalaldehyde-2-mercaptoethanol complex at 38oC to give a derivative fluorophor (post-column derivitization). The concentration of glyphosate eluted from the HPLC column is measured by detecting the fluorescence of the derivative with a fluorometer.

This method determines glyphosate in drinking water.

Methods for the Determination of Organic Compounds in Drinking Water - Supplement II (EPA/600/R-92-129)

(A) Glassware contamination: Thoroughly clean glassware, including baking or solvent rinse.(B) Reagent contamination: Use high purity reagents.

QC Requirements:
Initial demonstration of laboratory capability, followed by analysis of laboratory reagent blanks (LRBs), laboratory fortified matrix samples, laboratory fortified blanks (LFBs), and QC samples. Initial demonstration of laboratory capability includes analysis of LFBs at an analyte concentration of 250 ug/L. A MDL also must be determined.

Maximum Holding Time:
14 days generally; up to 18 months for glyphosate in frozen samples.



Not specified in method. Range differs depending on matrix and instrumentation.

Precision and accuracy values were determined using single laboratory data from eight replicate analyses of reagent water fortified with glyphosate.

MDLs were determined using the standard deviation of replicate analyses of an analyte-fortified reagent water sample (25 ug/L of glyphosate) multiplied by the t-value for (# of samples - 1) degrees of freedom at the 99% confidence level. Detailed instructions for how MDLs are determined are found at 40 CFR part 136, Appendix B. NOTE: MDLs in ground water (GW) and dechlorinated tap water (TW) were also determined (GW = 9 ug/L, TW = 6 ug/L).

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Test Description:
Glyphosate by HPLC

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