EPA Method 554

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EPA Method 554:
DETERMINATION OF CARBONYL COMPOUNDS IN DRINKING WATER BY DINITROPHENYLHYDRAZINE DERIVATIZATION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Summary:
A measured volume of aqueous sample, approximately 100 mL, is buffered to pH 3 and the analytes are derivatized at 40?C for one hour with 2,4-dinitrophenylhydrazine (DNPH). The derivatives are extracted from the water by passing the sample through a series of three cartridges each of which contains 500 mg of a chemically bonded C18 organic phase (liquid-solid extraction, LSE). The solid sorbent cartridges are then eluted with 10 mL of ethanol. LSE disks may also be used as long as all the (QC) criteria specified in Section 9.0 of this method are met. Liquid chromatographic conditions are described which permit the separation and measurement of the carbonyl compounds in the extract by absorbance detection at 360 nm.

Scope:
This is a high performance liquid chromatographic (HPLC) method optimized for the determination of selected carbonyl compounds in finished drinking water and raw source water. The analytes applicable to this method are partitioned from the water onto a reverse phase C18 bonded silica packed cartridge, then eluted with ethanol. Liquid-solid extraction disks may also be used for this purpose.

Report Number:
600/R-92-129