EPA Method 6500

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EPA Method 6500:
DISSOLVED INORGANIC ANIONS IN AQUEOUS MATRICES BY CAPILLARY ION ELECTROPHORESIS

Summary:
Capillary ion electrophoresis is a free-zone electrophoretic technique optimized for the analysis of anions with molecular weights of less than 200. The anions migrate and are separated according to their mobility in the electrolyte when an electrical field is applied through the open tubular fused silica capillary. The electrolyte's electroosmotic flow (EOF) modifier dynamically coats the inner wall of the capillary, changing the surface to a net positive charge. This reversal of wall charge reverses the natural EOF. The modified EOF in combination with a negative power supply augments the mobility of the analyte anions towards the anode and detector, achieving rapid analysis times. Cations migrate in the opposite direction towards the cathode and are removed from the sample during analysis. Water and other neutral species move toward the detector at the same rate as the EOF. The neutral species migrate slower than the analyte anions and do not interfere with anion analysis.

Scope:
This test method is applicable for determination of the dissolved inorganic anions in aqueous matrices (drinking water, wastewater, and ground water) using capillary ion electrophoresis with indirect UV detection.

Report Number:
SW-846 Ch 3.3