EPA Method 830.7570

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EPA Method 830.7570:
Partition Coefficient (n-Octanol/Water), Estimation By Liquid Chromatography

Summary:
HPLC is performed on analytical columns packed with a commercially available solid phase containing long hydrocarbon chains (e.g. C8, C18) chemically bound onto silica. Chemicals injected onto such a column move along it by partitioning between the mobile solvent phase and the hydrocarbon stationary phase. The chemicals are retained in proportion to their hydrocarbon-water partition coefficient, with water-soluble chemicals eluted first and oil-soluble chemicals last. This enables the relationship between the retention time on a reverse-phase column and the n-octanol/water partition coefficient to be established.

Scope:
This guideline is intended to meet testing requirements of both the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) (7 U.S.C. 136, et seq.) and the Toxic Substances Control Act (TSCA) (15 U.S.C. 2601).