EPA Method 8310

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EPA Method 8310:
Polynuclear Aromatic Hydrocarbons

Method 8310 provides high performance liquid chromatographic (HPLC) conditions for the detection of ppb levels of certain polynuclear aromatic hydrocarbons. Prior to use of this method, appropriate sample extraction techniques must be used. A 5- to 25-uL aliquot of the extract is injected into an HPLC, and compounds in the effluent are detected by ultraviolet (UV) and fluorescence detectors.

Method 8310 is used to determine the concentration of certain poly-nuclear aromatic hydrocarbons (PAH) in ground water and wastes.

SW-846 Online: Test Methods for Evaluating Solid Waste, Physical/Chemical Methods

(A) Solvents, reagents, glassware, and other sample processing hardware may yield discrete artifacts and/or elevated baselines, causing misinterpretation of the chromatograms. Analysis of method blanks is necessary. Specific selection of reagents and purification of solvents by distillation in all-glass systems may be required.(B) Interferences coextracted from the samples will vary considerably from source to source. Although a general cleanup technique is provided as part of this method, individual samples may require additional cleanup approaches.(C) The chromatographic conditions described allow for a unique resolution of the specific PAH compounds covered by this method. Other PAH compounds, in addition to matrix artifacts, may interfere.

QC Requirements:
See Method 8000 for information

Maximum Holding Time:
Aqueous samples - 7 days (sample), 40 days (extract). Solid samples - 14 days (sample), 40 (extract). Also, see SW-846 Chapter Four for information.



None given.


Method detection limits for naphthalene, acenaphthylene, acenaphthene, and fluorene are based on UV-detection, while those listed for other compounds are based on fluorescence detection.

Revision Number:
Revision 0; September 1986

Test Description:
Polynuclear Aromatic

Report Number:
SW-846 Ch 4.3.3

Instrument used for this test: