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View Actual EPA Method 9131 (PDF File)
EPA Method 9131:TOTAL COLIFORM: MULTIPLE TUBE FERMENTATION TECHNIQUE
Summary:The multiple-tube fermentation technique is a three-stage procedure in which the results are statistically expressed in terms of the Most Probable Number (MPN). These stages -- the presumptive stage, confirmed stage, and completed test -- are briefly summarized below. (For the analysis to be accurate, a five-tube test is required.)
Scope:This method is used to determine the presence of a member of the coliform group in ground water and surface water.
Report Number:SW-846 Ch 5