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EPA Method 504.1:

1,2-Dibromoethane (EDB), 1,2-Dibromo-3-Chloropropane (DBCP), and 1,2,3- Trichloropropane (123TCP) in Water by Microextraction and Gas Chromatography – Revision 1.1.

A 35-mL sample is extracted with 2-mL of hexane. The concentrations of analytes in the extract are measured using a gas chromatography (GC) system equipped with a linearized electron capture detector (ECD).

This method determines EDB, DCBP, and 123TCP (analytes) in finished drinking water and groundwater.

Methods for the Determination of Organic Compounds in Drinking Water – Supplement III (EPA/600/R-95-131)

(A) Solvent contamination: Use high purity solvents, and test solvents to prevent contamination.(B) Extracted interferences: Interference can occur from extracted non-target compounds, with retention times similar to target compounds.(C) DBCM Contamination: DBCM is a common disinfection byproduct which elutes near the EDB retention time window, and can obscure the EDB signal.

QC Requirements:
Initial demonstration of laboratory capability, followed by field reagent blanks (FRB), laboratory reagent blanks (LRB), laboratory fortified blanks (LFB), laboratory fortified sample matrix (LFM), and quality control samples (QCS). A MDL for each analyte must also be determined.

Maximum Holding Time:
14 days; extracts up to 24 hours.



0.03 – 200 ug/L

Precision and accuracy values were calculated from seven replicate analyses of fortified reagent water in a single laboratory. Additional data are available in the method.

MDLs were determined using the standard deviation of 8 replicate analyses of an analyte-fortified reagent water sample multiplied by the t-value for 7 degrees of freedom at the 99% confidence level. Detailed instructions for how MDLs are determined are found at 40 CFR part 136, Appendix B.

Revision Number:
Revision 1.1, 1995

Test Description:
EDB, DBCP, 123TCP by

Report Number:

Instrument used for this test: